Dioxygenase that catalyzes the 2-oxoglutarate-dependent selective hydroxylation of free L-proline to cis-3-hydroxy-L-proline (cis-3-Hyp). D-proline, trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, cis-4-hydroxy-D-proline, and 3,4-dehydro-DL-proline are not substrates. Source: Recombinant protein corresponding to aa1-290 from Streptomyces sp. L-proline cis-3-hydroxylase 1, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~44.0kD Amino Acid Sequence: MRSHILGRIELDQERLGRDLEYLATVPTVEEEYDEFSNGFWKNIPLYNASGGSEDRLYRDLEGSPAQPTKHAEQVPYLNEIITTVYNGERLQMARTRNLKNAVVIPHRDFVELDRELDQYFRTHLMLEDSPLAFHSDDDTVIHMRAGEIWFLDAAAVHSAVNFAEFSRQSLCVDLAFDGAFDEKEAFADATVYAPNLSPDVRERKPFTKEREAGILALSGVIGRENFRDILFLLSKVHYTYDVHPGETFEWLVSVSKGAGDDKMVEKAERIRDFAIGARALGERFSLTTW Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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