Metallo-beta-lactamase Type 2, Recombinant, Klebsiella pneumoniae, aa29-270, His-Tag

Product Overview

• Article Name: Metallo-beta-lactamase Type 2, Recombinant, Klebsiella pneumoniae, aa29-270, His-Tag
• Biozol Catalog Number: USB-585376
• Supplier Catalog Number: 585376
• Alternative Catalog Number: USB-585376-20,USB-585376-200
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin. Source: Recombinant protein corresponding to aa29-270 from Klebsiella pneumoniae Metallo-beta-lactamase type 2, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~29.6kD Amino Acid Sequence: GEIRPTIGQQMETGDQRFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHSLTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 29.6
• UniProt: C7C422
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.