Disulfide Oxidoreductase, Recombinant, E. coli (dsbA)

Product Overview

• Article Name: Disulfide Oxidoreductase, Recombinant, E. coli (dsbA)
• Biozol Catalog Number: USB-D3778-01
• Supplier Catalog Number: D3778-01
• Alternative Catalog Number: USB-D3778-01-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids). Recombinant protein corresponding to DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques. Molecular Weight: ~21.1kD (189 amino acids) Amino Acid Sequence: AQYEDGKQYT/ TLEKPVAGAP/ QVLEFFSFFC/ PHCYQFEEVL/ HISDNVKKKL/ PEGVKMTKYH/ VNFMGGDLGK/ DLTQAWAVAM/ ALGVEDKVTV/ PLFEGVQKTQ/ TIRSASDIRD/ VFINAGIKGE/ EYDAAWNSFV/ VKSLVAQQEK/ AAADVQLRGV/ PAMFVNGKYQ/ LNPQGMDTSN/ MDVFVQQYAD/ TVKYLSEKK Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 21.1
• Purity: 95% by SDS PAGE
• Form: Supplied as a liquid in 20mM Tris pH 7.5, 2mM EDTA