Epidermal Growth Factor, Recombinant, Human (EGF, beta-Urogastrone, URG)
Biozol Catalog Number:
USB-E3412
Supplier Catalog Number:
E3412
Alternative Catalog Number:
USB-E3412-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human. Source: EGF1-52 Biological Activity: The mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells. Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified. 97% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis
Form:
Supplied as a lyophilized powder in PBS
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