Growth Hormone, Recombinant, Human, 20kD Variant, aa27-202 (hGH)

Product Overview

• Article Name: Growth Hormone, Recombinant, Human, 20kD Variant, aa27-202 (hGH)
• Biozol Catalog Number: USB-G8997
• Supplier Catalog Number: G8997
• Alternative Catalog Number: USB-G8997-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

The 20kD variant form of human growth hormone (20kD hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kD, 191 amino acids) by the deletion of amino acid residues 32-46. The physiological role of 20kD hGH remains to be determined partly but it regards the main function as stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant protein corresponding to aa27-202 of human growth hormone, expressed in E. coli. Uniprot/Accession: NP_072053.1 Amino Acid Sequence: MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF Biological Activity: Measured in cell proliferation assay using Nb2-11 rat lymphoma cells. The ED50 for this effect was 1ng/ml. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 20
• NCBI: 072053
• UniProt: P01241
• Purity: 95% by SDS PAGE under reducing conditions and visualized by coomassie blue stain. Endooxin: 1EU/ug (LAL)
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.