Applications: Suitable for use in ELISA, Dot Blot, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:2500 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
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