Interleukin-11 (IL-11) is a potent lymphoid cell growth factor which stimulates the growth and survivability of certain B cells and T cells. Human IL-11 is a 19.1kD protein containing 178 amino acid residues. Recombinant protein corresponding to human IL-11 expressed in E. coli. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine T11 cells is 2ng/ml, corresponding to a specific activity of 5x10e5 units/mg. Amino Acid Sequence: MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
