Keratinocyte Growth Factor (KGF) is a potent mitogen for keratinocytes and epithelial cells. Human KGF is a 18.9kD protein containing 163 amino acid residues. Biological Activity: ED50 was determined by the dose- dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells to be 10ng/ml, corresponding to a specific activity of 1x10e5 units/mg. Amino Acid Sequence: CNDMTPEQM ATNVNCSSP ERHTRSYDY MEGGDIRVR RLFCRTQWY LRIDKRGKV KGTQEMKNN YNIMEIRTV AVGIVAIKG VESEFYLAM NKEGKLYAK KECNEDCNF KELILENHY NTYASAKWT HNGGEMFVA LNQKGIPVR GKKTKKEQK TAHFLPMAI T Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
18.9
Purity:
~90% by SDS-PAGE and HPLC Endotoxin: 0.1ng/ug
Form:
Supplied as a lyophilized powder from PBS, pH 7.0. Reconstituted with PBS to 0.1-1mg/ml. Do not vortex. This solution can then be diluted into other aqueous solutions.
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