Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5), IgG2b, Clone: [9H158], Rat, Monoclonal

Product Overview

• Article Name: Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5), IgG2b, Clone: [9H158], Rat, Monoclonal
• Biozol Catalog Number: USB-M2412-50D
• Supplier Catalog Number: M2412-50D
• Alternative Catalog Number: USB-M2412-50D-100
• Manufacturer: US Biological
• Host: Rat
• Category: Antikörper
• Application: ELISA, IP, WB
• Immunogen: Recombinant protein corresponding to Met1-Pro375 of human Maspin expressed in E. coli. Species Sequence Homology: chimpanzee, 99%, canine, 92%, mouse, 89%, rat, 88%, chicken, 64%

Product Description

Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. Applications: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit for recombinant human Maspin is ~100ng/lane under non-reducing conditions and 25ng/lane under reducing conditions. Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with recombinant human Maspin Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Maspin is ~15ng/well Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [9H158]
• Isotype: IgG2b
• NCBI: 002630
• UniProt: P36952
• Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Form: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.