Nerve Growth Factor, pro, Recombinant, Human (ProNGF)
Biozol Catalog Number:
USB-N2350-09H
Supplier Catalog Number:
N2350-09H
Alternative Catalog Number:
USB-N2350-09H-2,USB-N2350-09H-10
Manufacturer:
US Biological
Category:
Molekularbiologie
ProNGF is the pro-form of the neurotrophin nerve growth factor. Like the mature protein proNGF is characterized by the cysteine knot motif consisting of three cysteine bridges. The protein predominantly exists as a non-covalently linked homodimer. The proNGF is a 50kD dimer in solution, on SDS-PAGE monomer at about 25-30kD is detected. To analyze this protein on an SDS-PAGE., DTT as a reduction agent is absolutely necessary. Recombinant protein corresponding to human Pro-NGF produced in E. coli Amino Acid Sequence: MEPHSESNVPAGHTIPQVHWTKLQHSLDTALRRARSAPAAAIAARVAGQTRNITVDPRLFKKRRLRSPRVL FSTQPPREAADTQDLDFEVGGAAPFNRTHRSKRSSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVM VLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTA CVCVLSRKAVR. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
49.74
Purity:
95% (SDS-PAGE, RP-HPLC)
Form:
Supplied as a liquid in PBS, pH 7.2.
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