Phosphodiesterase 4A1A, Recombinant, Human, GST-Tag (PDE4A1A)

Product Overview

• Article Name: Phosphodiesterase 4A1A, Recombinant, Human, GST-Tag (PDE4A1A)
• Biozol Catalog Number: USB-P4072-03A
• Supplier Catalog Number: P4072-03A
• Alternative Catalog Number: USB-P4072-03A-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Human PDE4A1A (GenBank Accession No. U97584), full length protein with N-terminal GST tag (MW = 100kD), expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: Lot Specific. Sample Lot Value: 4,176 pmol/min/ug. Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37C. Assay Conditions: 10mM Tris-HCl, pH 7.4, 10mM MgCl2, 1mM MnCl2, 200uM cAMP, and 0.2ng/ul PDE4A1A at 37C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Product Properties

• Molecular Weight: 100
• NCBI: 97584
• UniProt: P27815
• Purity: >70% by SDS-PAGE
• Form: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT and 20% glycerol