PKM2, N-terminal HIS-Tag, Recombinant, Mouse (Pyruvate Kinase, Isozymes M1/M2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormon

Product Overview

• Article Name: PKM2, N-terminal HIS-Tag, Recombinant, Mouse (Pyruvate Kinase, Isozymes M1/M2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormon
• Biozol Catalog Number: USB-P9545-33
• Supplier Catalog Number: P9545-33
• Alternative Catalog Number: USB-P9545-33-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Source: Recombinant corresponding to aa2-end of mouse rPKM2 with N-terminal His tag expressed in E. coli (NM_011099). Specific Activity: 11pmol/min/ug. A 50ul PKM2 reaction is conducted in a buffer containing 50mM Tris, pH 7.4, 100mM magnesium chloride, 250mM potassium chloride, 40mM ADP, 100mM phosphoenolpyruvate (PEP) at RT for 15 minutes. ATP production is detected using Kinase-Glo Luminescent Kinase Assay Platform. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70C. Aliquots are stable for 6 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 58
• NCBI: 011099
• UniProt: P52480
• Purity: Purified 90%
• Form: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 3mM DTT, 20% glycerol.