VH1-Related Phosphatase, Vaccinia Virus (VHR, DUSP3), IgG2a, Clone: [8H126], Mouse, Monoclonal
Catalog Number:
USB-V2210-03
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| Article Name: | VH1-Related Phosphatase, Vaccinia Virus (VHR, DUSP3), IgG2a, Clone: [8H126], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-V2210-03 |
| Supplier Catalog Number: | V2210-03 |
| Alternative Catalog Number: | USB-V2210-03-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | WB |
| Immunogen: | Recombinant protein corresponding to the full length sequence of human VHR expressed in E. coli. |
| Vaccinia Virus VH1-related phosphatase (VHR), also known as dual-specificity phosphatase 3 (DUSP3), dephosphorylates a variety of substrates at serine, threonine, and tyrosine residues. VHR has been shown to dephosphorylate the mitogen-activated protein kinases ERK1, ERK2, and JNK, but not p38. Phosphorylation of VHR at Y138 by ZAP70 enhances the inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor signaling. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml with lysates of TE-85 human osteogenic sarcoma cell line, TF-1 human erythroleukemic cell line, and C2C12 mouse myoblast cell line. Conducted under reducing conditions. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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