SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9], IgG1, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9], IgG1, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX-GTX632604
• Hersteller Artikelnummer: GTX632604
• Alternativnummer: GTX632604-25,GTX632604-100
• Hersteller: GeneTex International
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IP, ICC, FACS, IHC-P, IHC, WB, ELISA
• Spezies Reaktivität: SARS Coronavirus 2, SARS Coronavirus
• Immunogen: The immunogen used to generate this antibody corresponds to SARS-CoV Sdelta10 (within S2 domain) protein (1029-1192 a.a.).
• Konjugation: Unconjugated
• Alternative Synonym: COVID-19, COV-2, COVID, SARS-COV-2, COV2, COVID-19 Spike, 2019-nCoV Spike, COVID19, SARS-CoV-2 Spike, SARS-COV, NCOV, CORONA

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: 1A9
• Isotyp: IgG1
• Puffer: PBS
• Reinheit: Affinity purified by Protein G.
• Lagerung: 2°C to 8°C, -20°C or -80°C

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: Assay dependent. FACS: Assay dependent. IP: Assay dependent. ELISA: Assay dependent. Sandwich ELISA: Assay dependent. IHC-P (cell pellet): Assay dependent. Capture : GTX632604, Detection: GTX635654/GTX135356
• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: Assay dependent. FACS: Assay dependent. IP: Assay dependent. ELISA: Assay dependent. Sandwich ELISA: Assay dependent. IHC-P (cell pellet): Assay dependent. Capture : GTX632604, Detection: GTX635654/GTX135356/

Bilder

FACS analysis of 293T cells infected with recombinant vaccinia virus carrying the Spike gene using GTX632604 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9].

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV Spike protein by western blot analysis. Non-transfected (-) and SARS-CoV Spike-transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis.
Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone.
Green: SARS-CoV-2 infected Vero E6 cells stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Blue: Nuclei were counterstained with DAPI.

*From Zheng Z, et al. bioRxiv(2020). Shown under license agreement

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2-transfected COS-7 cells were fixed in 4% paraformaldehyde for 10 min at room temperature.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Blue: Nuclei were counterstained with DAPI.

*From Zheng Z, et al. bioRxiv(2020). Shown under license agreement

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135386 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135360 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Immunoprecipitation of SARS-CoV-2 Spike transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) at 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed in 4% paraformaldehyde at RT for 30 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000.

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco-2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635672 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135356 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Red: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635654 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min