QuickSwitch™

Custom Tetramer Kits from MBL

MBL offers novel solutions for screening MHC binding peptides and creating custom MHC tetramers. Functional screening of peptides for MHC class I binding is essential for vaccine development and immune system monitoring.

QuickSwitch™ Custom Tetramer Kits

Preparation of custom tetramers in the laboratory using MBL's proprietary peptide exchange technology.

Features

Prepare custom tetramers in 4 hours* (*Reaction time is different for each kit.)
No UV lamp or special instrument required
Quantify peptide exchange efficiency (Quant Tetramer Kits)
Select ready-to-use tetramer in PE, APC, or BV421

Principle of the peptide exchange reaction

MHC tetramers in QuickSwitch™ Custom Tetramer Kits are pre-bound with "exiting peptide" (shown in blue) to maintain structural integrity. Exchange of the exiting peptide with experimental peptide (shown in red) is initiated upon addition of the experimental peptide and a peptide-exchange factor (reaction time is 4 hours~*). The efficiency of peptide exchange depends on the sequence of the experimental peptide. QuickSwitch™ Quant Tetramer Kits contain reagents for determination of the peptide exchange efficiency.
*Reaction time is different for each kit.

Workflow

Peptide exchange, quantification, cell staining, and flow cytometry analysis can all be performed in one day:

*Reaction time is different for each kit.

Determination of peptide exchange efficiency

QuickSwitch™ Quant Tetramer Kits contain FITC labeled antibody which detects to the exiting peptide pre-bound to MHC molecules. After the peptide-exchange reaction, the MHC tetramers are adsorbed to special beads and reacted with the antibody for the determination of peptide exchange efficiency by flow cytometry.

Product Name

Art. N°

PE-labeled

APC-labeled

BV421-labeled

QuickSwitch™ Quant HLA-A*01:01 Tetramer Kit