Streptavidin Coated PCR-Tubes and Microplates

for DNA-assisted immunoassays

Newest epitope detection methods as immuno PCR assays, or DNA-assisted immunoassays combine the high specifity of antibody binding with the enormous amplification capacity of the polymerase chain reaction (PCR). Affinity probes with conjugated amplifiable oligonucleotides allow proteins to be detected at the level of single molecules or molecule complexes.

Together with our partner Biomat, specialst for surface modifications, we provide you with Streptavidin coated PCR tubes and microplates, which enable binding of biotinylated molecule complexes directly in the well for further PCR amplification.

Streptavidin coated surfaces offer a powerful and universal instrument for binding any biotinylated molecule (Proteins, Peptides, Polysaccharides, Oligonucleotides, DNA fragments etc.). Streptavidin is a tetrameric protein (M.W. 60 kDa) with very high affinity for biotin (Ka=10- 15 M); the bond is the strongest known non-covalent biological interaction.

The streptavidin-biotin bonding main features are:

stability
specificity
affinity

This feature make this coating suitable for special applications of molecules which do not offer reliable bonding by passive adsorption or adsorb in a unfavorable orientation.

Biomats streptavidin coated PCR products were applied and cited in the work of Wu et al., who profiled surface proteins on individual exosomes.

Biomat streptavidin coated PCR tubes and microplates:

BMT-PCR0STF-SA5/100	Streptavidin Coated PCR Tubes
BMT-PCR96010STF-SA5/100	Streptavidin PCR Plates, non-skirted
BMT-PCR96020STF-SA5/100	Streptavidin PCR Plates, semi-skirted
BMT-PCR96030STF-SA5/100	Streptavidin PCR Plates, skirted