New from Atlas Antibodies: MolBoolean™

Unlock the secrets of protein-protein interactions

Atlas AntibodiesAtlas Antibodies is the original manufacturer of highly validated primary antibodies and control antigens. The company was founded by researchers from the prestigious Human Protein Atlas project who wanted to make the unique antibodies developed in the project available to fellow researchers worldwide.

With MolBoolean™ Atlas Antibodies now unveils a revolutionary molecular tool: a groundbreaking in situ proximity technology designed to revolutionize protein analysis.

MolBoolean - Beyond Proximity Ligation

Unlock the secrets of protein-protein interactions in cells and tissue with MolBoolean™

MolBoolean™ is a novel in situ proximity technology developed by Atlas Antibodies that enables the simultaneous detection of both free and interacting fractions for two protein targets.

Traditional approaches to study protein-protein interactions (PPIs) in cells and tissue could generate unreliable results due to the need of using engineered protein expression systems or lysed cell material. The introduction of in situ proximity ligation assay (in situ PLA) enabled the detection of endogenous PPIs in cells and tissue but it still lacks information on the fraction of non-interacting proteins.

The newly developed MolBoolean™ technology from Atlas Antibodies relies on dual target recognition with proximity probes and generates rolling circle amplification products to increase signals, meaning that even low abundant proteins can be detected. On top of that, by performing a series of proprietary molecular steps, MolBoolean™ differentiates between amplified products stemming from individual versus interacting proteins, and this is indicated by the binding of one or two separate fluorescent detection reporters. It can be used for basic research or in drug discovery to study cell signaling PPIs in health and disease and how these may be modified by drug intervention.

Features: 

  • Complete spatial quantitative analysis of protein-protein interactions by simultaneous detection of free and interacting proteins. 
  • Accurate quantification by normalization of interaction data to total target protein levels. 
  • Biologically relevant data without the need for engineered protein expression.
  • 1000-fold increased fluorescence signal by Rolling Circle Amplification, allowing detection and quantification of low abundant proteins.  
  • Adaptable to different research needs. Universal kit that can be used with the customer’s choice of primary antibodies. 
  • Validated in both cells and tissue.

 

MolBoolean™ Kit (Art. N° ATA-MOLB00001)

Product Data SheetProduct Safety Data Sheet

Included in the Kit: Antibody proximity probes, oligonucleotides, enzymes, fluorescent detection reporters.
Not included in the Kit: Primary antibodies.
Target Species: Mouse, Rabbit.
Kit Size: 4.8 ml, approximately 120 assays (40 µl/assay).

The MolBoolean kit is available at an introductory price of € 1.995,00 until the end of the year.

 

Launch celebration offer:

Until end of the year, with every purchased MolBoolean Kit, you can purchase

antibodies from Atlas Antibodies used with the kit with a 50 % discount.

 

EMD/LMNB1 MolBoolean staining in MCF7 cells.

MolBoolean™ analysis of the interaction between EMD (magenta) and LMNB1 (green) in MCF7 cells, using the monoclonal anti-EMD (Art. N° ATA-AMAb90562) and the polyclonal anti-LMNB1 (Art. N° ATA-HPA050524) antibodies from Atlas Antibodies AB.

Image shows the spatial location of free versus interacting protein fractions, indicated by the detection of rolling circle products (RCPs) in either one or two fluorescent channels.

Learn more from Atlas Antibodies' Application Note MolBoolean (pdf).

 

08.05.2024

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