Ex vivo models

Why in vivo antibodies are a natural fit

For over 20 years, scientists have trusted Bio X Cell as their go-to source for in vivo functional grade antibodies, backed by over 20,000 peer-reviewed publications citing Bio X Cell products. Bio X Cell offers 300 high-quality, pure in vivo monoclonal antibodies covering a broad spectrum of your research needs. Learn why these in vivo antibodies are a natural fit for ex vivo applications.

Why InVivo Formulation Antibodies Are a Natural Fit

Technologies such as artificial intelligence, organ-on-a-chip systems, and in silico modeling are transforming early-stage drug discovery. Regulatory bodies including the FDA and NIH are increasingly supporting validated alternatives to animal testing where appropriate. As researchers accelerate the shift toward human-relevant, non-animal systems, ex vivo models are taking center stage in drug discovery and translational science. Scientists working with sensitive NAM (New Approach Methodology) assays such as organoids, organs-on-chip, 3D cell cultures, and traditional cell and tissue culture, require antibodies that meet exceptional standards of purity, consistency, and performance to ensure low cytotoxicity, low off target effects, and to minimize inconsistent experimental results.

In vivo formulated antibodies are able to help researchers bridge NAM studies with traditional in vivo work to provide a comprehensive research workflow moving from tissue systems to animal models, and back again as needed in a flexible approach to the pre-clinical workflow.

What Are Ex Vivo Models?

Ex vivo models are biological systems maintained or grown outside of an organism but which retain key features of desired in vivo functions. Ex vivo models include:

Organoids – iPSC derived mini-organs that model the architecture and function of whole-organism tissues
Organs-on-Chip – Microengineered systems that simulate organ-level physiology using live cells in dynamic environments
3D Cell Cultures – Structures that replicate tissue-like complexity for more predictive biology
Traditional Cell & Tissue Culture – Well-established models for studying cellular mechanisms in controlled environments

These models are central to New Approach Methodologies (NAMs) and are transforming the landscape of immunology, oncology, neuroscience, and toxicology research.

Why In Vivo-Formulated Antibodies Excel in Ex Vivo Systems

Bio X Cell’s antibodies, originally designed for in vivo applications, feature qualities that naturally support the demands of ex vivo model systems, where sensitivity, reproducibility, and biological relevance are critical.

1. Ultra-Pure, Carrier-Free Formulation

Ex vivo systems are highly sensitive to formulation variables. Antibodies free from preservatives, stabilizers, and carrier proteins ensuring minimal background and clean, interpretable data.

2. Low Endotoxin Levels

Endotoxins can disrupt cellular responses in ex vivo models. Ultrapure, low endotoxin antibodies are rigorously tested to help maintain biological integrity and reproducibility in sensitive cell-based systems.

3. Lot-to-Lot Consistency

Consistency is everything in data-driven platforms like organoids and organs-on-chip. Lot-to-lot reproducibility is a critical component of selecting research reagents for these systems to ensure confidence across replicates, labs, and time.

Flexibility Across Models = Efficiency in Discovery

One of the key advantages of using Bio X Cell functional antibodies is their cross-platform compatibility. The same antibody can be used in an organoid or organ-on-chip study then can be applied directly in an in vivo mouse model, without reformulation or revalidation. This flexibility streamlines workflows and eliminates the need to source multiple versions of the same antibody for different systems.

This is especially beneficial for:

Validating early-stage findings in ex vivo models and seamlessly translating them to in vivo studies

Reducing variability and complexity in reagent sourcing

Saving time and resources by minimizing method development for each new model system

For researchers working across both NAMs and traditional in vivo studies, Bio X Cell antibodies offer a rare combination of precision, purity, and versatility.

As ex vivo models become more sophisticated and central to discovery workflows, researchers need tools that keep pace. Bio X Cell’s in vivo formulation antibodies offer the quality, consistency, and performance needed to power the next generation of human-relevant science, while maintaining the flexibility to scale into in vivo models for deeper insights.