Autophagy Research

Gold standard Anti-LC3 antibodies from MBL Int.

Autophagy refers to the process of degradation and utilization of own cellular components such as misfolded proteins or organelles by eukaryotic cells. Autophagy in mammals is involved not only in the starvation response, but also in antigen presentation, cell death, development, aging, tumorigenesis, and defense against bacterial infections. Autophagy research is therefore important for understanding all these processes.

LC3 has been studied extensively and is used as an autophagy marker in mammals. Soluble LC3  is distributed ubiquitously in mammalian tissues and cultured cells.  During autophagy, autophagosomes engulf cytoplasmic components, and LC3-II (LC3-phosphatidylethanolamine conjugate) is recruited to autophagosomal membranes. As autophagosomes fuse with lysosomes, and intra-autophagosomal components are degraded by lysosomal hydrolases, LC3-II in autolysosomal lumen is degraded. Thus, lysosomal turnover of the autophagosomal marker LC3-II reflects starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death.

In order to determine the levels of autophagy, our partner MBL provides you with validated monoclonal and polyclonal Anti-LC3 antibodies for immunoblotting, immunoprecipitation, and immunofluorescence.

 

Selected Anti-LC3 antibodies from MBL Int.:

Product Code	Product Name	Species Reactivity	Applications
MBL-M152-3	Anti-LC3 Monoclonal Antibody	Human, Mouse, Rat, Hamster	FCM, ICC, IP, WB
MBL-M186-3	Anti-LC3  Monoclonal Antibody	Human, Mouse, Rat, Hamster	WB
MBL-M186-7	Anti-LC3 Monoclonal Antibody HRP-DirecT	Human, Mouse, Rat, Hamster	WB
MBL-PD014	Anti-LC3 Polyclonal Antibody	Human, Mouse, Rat, Hamster	WB
MBL-PM036	Anti-LC3 Polyclonal Antibody	Human, Mouse, Rat, Hamster	FCM, ICC, IHC, IP, WB